In today’s contemporary business environment, the issue of work stress has become a matter of debate and general interest amongst researchers. This is because work stress connotes harmful physical and emotional responses that occur when job requirements do not match worker’s capabilities and resources with adverse effect on service delivery if not properly handled. Hence, this study examined the impact of work stress on internal customer service satisfaction in Benson Idohosa University, Benin- city, Edo State. The specific aspects of job stress examined are work overload, role ambiguity and role conflict. A descriptive survey was adopted as the foci of the research design. The population of this study comprised 153 employees of three faculties in Benson Idahosa University, while a sample size of One Hundred and Eleven (111) was systematically selected using Yamane (2002). Based on this, a structured questionnaire was designed and administered to elicit information from the respondents. Out of One Hundred and Eleven (111) copies of questionnaire administered, 109 were retrieved and found useable for final data analysis. Multiple regression analysis was employed to confirm the significant impact of work stress on internal customer service satisfaction among employees of Benson Idahosa University. The results revealed that work overload and role conflict have no significant influence on the quality of internal customer services satisfaction received, but role ambiguity has a significant effect on the quality of internal customer services satisfaction received. The findings underscored the importance of reducing and managing work overload, role ambiguity and role conflict. This study, therefore, recommended that Benson Idahosa University should make use of proactive management support, partnership process and improving internal communications so as to enhance quality internal customer service satisfaction.